DNA

Part:BBa_M36656:Design

Designed by: Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-30)


Aromatase Cytochrome P450 DNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1213
    Illegal PstI site found at 1387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1213
    Illegal PstI site found at 1387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1213
    Illegal BglII site found at 707
    Illegal BglII site found at 867
    Illegal XhoI site found at 1462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1213
    Illegal PstI site found at 1387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1213
    Illegal PstI site found at 1387
    Illegal NgoMIV site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted this part into an HEK293t mammalian vector. Other than placing this gene after a promoter region in our vector, we didn't have anything in particular to change or consider when designing.


Source

Genomic sequence is encoded in human DNA and is provided by the National Center for Biotechnology Information

References